Genesee & Wyoming Australia Pty Ltd was formed in June 2006 after GWI and its Australian joint venture partner, Wesfarmers Limited, sold their Western Australia operations and certain other assets of the Australian Railroad Group (ARG). GWI purchased Wesfarmers' 50 percent ownership interest in ARG's remaining operations, which are principally located in South Australia and the Northern Territory. The Adelaide-based business was renamed Genesee & Wyoming Australia Pty Ltd and is a 100 percent-owned subsidiary.
Genesee & Wyoming’s involvement in Australia started in 1997, when GWI Australia Pty. Ltd. acquired the assets of Australian National's freight rail business in the state of South Australia from the Commonwealth of Australia and created Australia Southern Railroad (ASR), a wholly owned subsidiary of GWI Australia. ASR's predecessor incurred substantial losses in 1996, yet, on the first day of operations, ASR turned the business into a profitable enterprise.
With the 2010 acquisition of the 2,200-km Tarcoola-to-Darwin railway, Genesee & Wyoming Australia Pty Ltd became the largest of the 11 Genesee & Wyoming operating regions around the world.